A Flexible DNA Biosensor for Breast Cancer Marker BRCA1 Detection
Wang, Q (Wang, Qi)[ 1,2 ] ; Zhang, HY (Zhang, Huanyu)[ 1 ] ; Shen, XH (Shen, Xiaohui)[ 1 ] ; Wang, FH (Wang, Fenghe)[ 3 ]* ; Mao, C (Mao, Chun)[ 1 ]*（毛春）
[ 1 ] Nanjing Normal Univ, Natl & Local Joint Engn Res Ctr Biomed Funct Mat, Sch Chem & Mat Sci, Nanjing 210023, Peoples R China
[ 2 ] Nanjing Normal Univ, Sch Geog, Nanjing 210023, Peoples R China
[ 3 ] Nanjing Normal Univ, Sch Environm, Jiangsu Prov Key Lab Environm Engn, Nanjing 210023, Peoples R China
CHEMISTRYSELECT 2020, 5, 15259–15263
Early detection of breast cancer is of great significance to improve the prognosis and survival rate of breast cancer. However, how to detect breast cancer 1 gene mutation (BRCA1) efficiently in early stage is still a great challenge. In the past few decades, more and more attention has been paid to the design of a biosensor. In this work, A method for rapid detection of specific BRCA1 using silver nanowires modified polyethylene terephthalate (PET) film as a disposable electrode was proposed. With a high aspect ratio, AgNWs distributed in the PET film were easy to form a conductive network as a flexible electrode. A well-conductive gold sputtering layer can prevent AgNW from falling off to provide a conductive and stable electrode substrate. The Au-HRP can effectively reflect the hybridization matching phenomenon of target. Results indicated this strategy we proposed had a low detection limit with different concentrations of complementary DNA with limit of detection of 5.3x10(-15) M in the range from 5.0x10(-10) M to 5.0x10(-7) M. Due to the high sensitivity, simplicity and cheap price, arbitrary cut, AgNWs/PET film becomes a promising electrode material for quantification of low concentration biological component in early diagnosis.